Monitoring behavioral and physiological responses: Independent and combined analysis

Relying on non-invasive methods, i.e., cameras installed at a distance and implanted communicating heart rate loggers, the multi-level response of a captive colony of Humboldt penguins was monitored without human disturbance when facing the approach of a non-human intruder, i.e. a rover (remotely operated vehicle).

Given the lack of investigations of these features in a predatory/intrusion context, the influence of the intruder's appearance, i.e. color, and its way of approach, i.e. continuous or discontinuous, was studied.<br><br>Eight datasets are provided in the 'final_data_files' folder, corresponding to specific aspects of the response analysis to thepresence of this intruder :</p><p dir="ltr">- 'behavior_final_data_2023.csv' summarizes behavioral observations from video recordings dated from 2023 coded tanks to the BORIS software (v7.10.2),<br>- 'behavior_pilot_data_2021.csv' summarizes behavioral observations from video recordings dated from 2021 coded tanks to the BORIS software (v7.10.2),<br>- 'delay-order_vigilance.csv' summarizes delay and order for first events of vigilance from video recordings dated from 2023 coded tanks to the BORIS software (v7.10.2),<br>- 'delay-order_flight.csv' summarizes delay and order for first events of flight from video recordings dated from 2023 coded tanks to the BORIS software (v7.10.2),</p><p dir="ltr">- 'overview_comp_duration.csv' compares the physiological stress duration assessed following the presence of the rover or a human inside the enclosure,<br>- 'overview_comp_stress.csv' compares mean and maximum heart rates recorded during rover experiments and anthropogenic presence,<br>- 'summary_data.csv' presents all the heart rate data collected across the rover experimental conditions, i.e. combination of color and approach pattern,<br>- 'final_concat_HR.csv' compiles behavioral and heart rate data to account for the effect of physical activity on heart rate.<br><br>Furthermore, the second folder 'rover_tests' contains elements to redo the analyses from scratch, including some video recordings of the ROV approach in 2023 and the penguin reactions, as well as a representative picture to identify each member of the colony.<br><br>Additional information concerning the structure of the datasets is available in the READ.me file.