Data · dataset · 2019
Microsatellite genetic characterization of Silene ciliata Poiret (Caryophyllaceae)
Listed in e-cienciaDatos
We collected S. ciliata leaf tissue from 20 genets per population for genetic analysis (n = 180).
Description
DNeasy Plant minikit (QIAGEN, Valencia, USA) was used for DNA extraction of 10–20 mg of dried S. ciliata tissue. Based on a previous study (García-Fernández et al. 2012) we selected eight microsatellite loci for genotyping: Sci1224, Sci1208, Sci0106, Sci1443, EST-2HTS, EST-37HTS, EST-G34D06 and EST-G47A02.
PCR protocols were performed as described in García-Fernández et al. (2012). We genotyped all samples in an automated DNA sequencer (ABI PRISM 3730, Applied Biosystems, California, USA) in Parque Científico de Madrid (Madrid, Spain). GeneMarker version 1.85 (SoftGenetics, StateCollege, Pennsylvania, USA) was used for fragment size determination.
Read the rest (1 more)
By re-amplifying and re-scoring 20% of the samples we evaluated genotyping accuracy
Links
Where it is published
- Dataverse dataset page edatos.consorciomadrono.es/dataset.xhtml?persistentId=doi%3A10.21950%2FGSTZ26 ↗
landing page · from edatos consorciomadrono es
- DOI doi.org/10.21950/gstz26 ↗
DOI / persistent id · from edatos consorciomadrono es
Catalogue records · 1
- Dataverse API edatos.consorciomadrono.es/api/datasets/:persistentId/?persistentId=doi%3A10.21950%2FGSTZ… ↗
metadata API · from edatos consorciomadrono es
Topics
- Stated by source
- Ciencias de la tierra y el medioambiente
- From keywords
- Life Sciences · Social Science
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| e-cienciaDatos | doi:10.21950/GSTZ26 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].dataverse_subject:ciencias-de-la-tierra-y-el-medioambiente | source · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 | /subjects |
| concepts[field].local:field:social-science | mapping · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 | /subjects |
| created_date | source · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 | |
| description | source · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 | /description |
| publication_date | source · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 | |
| title | source · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 | /name |
| updated_date | source · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 | |
| version_label | source · edatos consorciomadrono es | connector:edatos_consorciomadrono_es@1.0.0 |