Table · dataset · 2026
<p>Ppp1r3g interacts with 14-3-3 family proteins.</p>
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<div><p>Protein phosphatase 1 regulatory subunit 3G (PPP1R3G) is one of the regulatory subunits of protein phosphatase 1 (PP1).
Description
Current research on PPP1R3G is primarily focused on glycogen synthesis and lipid metabolism. However, the role of <i>ppp1r3g</i> in the innate immune response of fish has not been completely elucidated.
This study demonstrated that <i>ppp1r3g</i> regulates RLR signaling pathway through interactions with Rig-I, Mda5, and Traf6. In parallel, Ppp1r3g inhibits platelet activation by regulating 14-3-3 zeta (14-3-3ζ) and consequently modulating the formation of the 14-3-3ζ-integrin-β3 (Itgb3) complex. <i>In vivo</i> analysis revealed that compared with the wild-type controls, <i>ppp1r3g</i>-null rare minnow (<i>Gobiocypris rarus</i>) and <i>ppp1r3g</i>-chimera grass carp (<i>Ctenopharyngodon idella</i>) exhibited significantly enhanced resistance to grass carp reovirus (GCRV), with markedly alleviated hemorrhagic symptoms post-infection.
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Furthermore, knockdown of <i>ppp1r3g</i> suppressed GCRV replication and upregulated type I interferon (IFN-1) expression in grass carp kidney (CIK) cells. Additionally, Ppp1r3g binds to the -ESKVFYLKMKGDYYRYL- fragment (EL17) of 14-3-3ζ <i>via</i> the Ser36/37 phosphorylation site located on the PP1c domain. This study also demonstrated that 14-3-3ζ interacts with Itgb3 through this fragment, indicating that Ppp1r3g competes with Itgb3 to bind 14-3-3ζ.
Furthermore, Ppp1r3g dose-dependently promoted Itgb3 degradation via a proteolytic pathway. These findings indicate that Ppp1r3g negatively regulates innate antiviral immunity after GCRV stimulation and suppresses platelet and coagulation cascade activation by modulating the interaction between 14-3-3ζ and Itgb3 in fish.</p></div>
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Where it is published
- DOI doi.org/10.1371/journal.ppat.1014590.s009 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
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Provenance · 1 source records, 21 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| figshare | oai:figshare.com:article/33937336 | 10 d ago | JSON v1 |
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| concepts[field].anzsrc:group:3105 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3204 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
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| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
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| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| description | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/description |
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| title | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/title |