Data · dataset · 2022
Identification of Substrates of MBL Associated Serine Protease-1 (MASP-1) from Human Plasma using N-terminomics Strategy
Listed in Teesside University Research Data Repository
Human blood was drawn from healthy adult volunteers after taking their informed consent.
Description
The study was approved by the institutional ethics committee, Ref. No. All India Institute of Medical sciences (AIIMS) /Patna/Institutional Ethics Committee/2019/413.
All the experimental work was done in accordance with the relevant guidelines and regulations. Acid-citrate-dextrose (ACD) was used as an anticoagulant (6 vol. of blood to 1 vol. of ACD). Platelet-rich plasma was obtained by centrifuging the ACD blood at 200 g for 15 min.
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This was followed by plasma depletion of albumin and Immunoglobulin G (IgG) using antibody-based Abundant Protein Depletion Spin Columns (Pierce, Thermo Fisher, MA, USA). The antibodies were specific to the Fc domain of immunoglobulins.
Links
Where it is published
- DOI doi.org/10.17632/mr5h7xfknb.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Life Sciences · Physics
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/mr5h7xfknb.1 | 8 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:physics | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |