16S rRNA gene sequencing of non-lactic acid bacteria cultures from lait caillé in Burkina Faso

(GTG)5-based rep-PCR fingerprint followed by cluster analysis, sequencing of 16S rRNA gene and database search in GenBank were performed to identify the isolates. Data showed a predominance of Klebsiella pneumoniae/K. quasipneumoniae/K. variicola among Gram negative bacteria and a variety of species among Gram positive catalase positive bacteria such as Kocuria spp., Bacillus cereus group species, Macrococcus caseoliticus, Clostridium bifermentans, Staphylococcus hominis.

These data are useful as they gave some information on the presence of bacteria associated with emerging public health risks such as Klebsiella pneumonia species complex, Bacillus cereus group species, Kocuria spp. occurring in a traditional spontaneously fermented milk product. They also gave further insights for a broader investigation on non-lactic acid bacteria occurring in traditional fermentation of unpasteurised milks and for evaluation and development of selective media for Lactic acid bacteria growth.