Data for: Persistent and Compartmentalized T cell Immunity in ALS

The present data is on single-cell sequencing isolated from human cerebrospinal fluid (CSF) cells and blood samples from newly diagnosis ALS patients (n=17) and ALS follow up (n=4). This analysis was conducted as part of a bigger project which is summarized in the section below.

Using single-cell RNA and TCR sequencing in combination with imaging flow cytometry of cells derived from cerebrospinal fluid (CSF) and blood, we identified increased amount of antigen-presenting cell:T cell complexes in CSF, suggesting local activation. Clonal T cell expansion in CSF was observed with high inter-patient variability, indicating immunological heterogeneity among ALS patients. Notably, CMV-specific T cells in CSF, but not blood, positively correlated with the rate of disease progression, implicating a chronic viral component in ALS.