Data · dataset · 2023
Immune cell senescence and exhaustion
Listed in ScienceDB
Initially, we conducted wound healing tests on HCT116 cells, employing SDC1 or SDC4 knockdown.
Description
The extent of wound healing was assessed at 0h, 24h, and 48h, and wound healing percentages were calculated using ImageJ software (v1.54d). Subsequently, Transwell migration assays were carried out to evaluate the migratory capacity of HCT116 cells after the knockdown of SDC1 or SDC4.
Finally, q-PCR was conducted to measure the expression levels of EMT-related markers E-Cadherin (CDH1), N-Cadherin (CDH2), and Vimentin (VIM) in HCT116 cells following siRNA knockdown of SDC1 and SDC4.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.13041 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.13041 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |