Data · dataset · 2026
Macrophage sabotage: undermining macrophage signalling by Klebsiella pneumoniae
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32637717.v1
Klebsiella pneumoniae has been singled out as an urgent threat to human health due to the increasing number of multidrug resistant isolates.
Description
Notably, there are still significant knowledge gaps in our understanding of Klebsiella-innate immune system interface. In a previous work of the lab (Ivin et al., 2017), we demonstrated that type I IFN signalling plays an important role in host defence against the infection.
In this work, we aim to shed mechanistic light into the role of type I IFN signalling in macrophage intrinsic immunity.<br><br>Wild Type and Interferon α/β receptor knockout (Ifnar1-/-) Immortalised Bone Marrow Derived Macrophages (iBMDMs) cells were infected. Inflammatory signals were quantified and intracellular bacteria quantified by plating assays and single cell microscopy analysis.<br><br>We observed an impaired formation of the phagocytic cup in Ifnar1-/- macrophages due increased cofilin inactivation.
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Intriguingly, type I IFN signalling is essential for the intracellular survival of Klebsiella in macrophages. Lastly, an increase of inflammation was observed in infected Ifnar1-/- macrophages suggesting that type I IFN signalling modulates inflammatory responses following Klebsiella infection.<br>Our results show that type I IFN signalling is essential for phagocytosis as well as intracellular survival of Klebsiella pneumoniae.
We have also shown that interferon signalling is required for Klebsiella to limit inflammation. This work highlights the importance of using live pathogens to study immune responses as previous studies using Ifnar1-/- models have used LPS to simulate infection thus have missed this phenotype.<br>
Links
Where it is published
- DOI doi.org/10.17034/32637717.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Microscopy 75%
Provenance · 3 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32637717 | 11 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32637717 | 11 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32637717 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[modality].local:modality:microscopy | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |