Imaging · dataset · 2022
Dataset Figure_6: Cln3 levels pulse prior to Start
Listed in IISH Dataverse
Data and Matlab script 1.
Description
FLmean_Cln3_GLU: CSV file containing mean GFP concentration from Cln3-P2A-GFP for single daughter cells grown in glucose. Each cell is followed from birth until a few minutes after bud appearance.
Data is organized by columns (one column corresponds to one cell). The first column is the vector of measurement time points (in minutes). 2. Vol_Cln3_GLU: CSV file containing cell volume for each of the daughter cells described above.
Read the rest (2 more)
Data is organized by columns (one column corresponds to one cell). The first column is the vector of measurement time points (in minutes). 3. bud_times: Excel file containing the time of bud appearance for each of the daughter cells described above 4. GP_FLtot_Cln3_GLU_example: Matlab script used to perform Gaussian process regression (see description in Methods) on the total GFP fluorescence for each of the daughter cells described above.
The script produces single-cell Cln3 abundance data used to generate Fig. 5c of the main text and Extended Data Figure 4H. NOTE: in order to run, the Matlab script requires the installation of the GPML Matlab toolbox (freely available at gaussianprocess.org/gpml/code/matlab/doc/).
Links
Where it is published
- Dataverse dataset page dataverse.nl/dataset.xhtml?persistentId=doi%3A10.34894%2FPKZZOJ ↗
landing page · from datasets iisg amsterdam
- DOI doi.org/10.34894/pkzzoj ↗
DOI / persistent id · from datasets iisg amsterdam
Catalogue records · 1
- Dataverse API dataverse.nl/api/datasets/:persistentId/?persistentId=doi%3A10.34894%2FPKZZ… ↗
metadata API · from datasets iisg amsterdam
Topics
- Stated by source
- Medicine, Health and Life Sciences
- From keywords
- Life Sciences · Medicine & Health · Microscopy · Saccharomyces cerevisiae
- Inferred from text
- Biochemistry and cell biology 69% · Text 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| IISH Dataverse | doi:10.34894/PKZZOJ | 10 d ago | JSON v1 |
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| concepts[field].anzsrc:group:3101 | enrichment · datasets iisg amsterdam | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].dataverse_subject:medicine-health-and-life-sciences | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /subjects |
| concepts[modality].local:modality:microscopy | mapping · datasets iisg amsterdam | vocabulary-mapper@1.0.0 | keywords['microscopy'] |
| concepts[modality].local:modality:text | enrichment · datasets iisg amsterdam | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:4932 | mapping · datasets iisg amsterdam | vocabulary-mapper@1.0.0 | keywords['Saccharomyces cerevisiae'] |
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| description | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /description |
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| title | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /name |
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