Omics · study · 2026
Synthetic genome expansion reveals a transcriptional constraint on genome size
Listed in NCBI GEO
Genome sizes vary across eukaryotes, largely because of differences in non-coding DNA.
Description
However, the physiological effects of this excess DNA are unclear. We engineered budding yeast strains carrying up to 12.8 Mb of predominantly non-coding human DNA, doubling the genome without altering endogenous genes.
Genome expansion slowed growth and increased cell size. Spike-in-normalized ChIP-seq and RNA-seq showed that added DNA recruited RNA polymerase II, diverting it from endogenous genes and lowering endogenous mRNA concentration. Ribosome profiling and proteomics revealed little translation from added sequences.
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A model linked this transcriptional competition to reduced ribosome activity and growth. Thus, excess DNA imposes a fitness cost by sequestering transcriptional resources, providing a mechanistic basis for selection against genome expansion in rapidly proliferating organisms such as yeasts and bacteria.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346640 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346640 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1526114 ncbi.nlm.nih.gov/bioproject/PRJNA1526114 ↗
project · from NCBI GEO
Topics
- Stated by source
- Genome binding/occupancy profiling by high throughput sequencing · Saccharomyces cerevisiae
- From keywords
- Life Sciences
- Inferred from text
- Mass spectrometry 65% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346640 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:mass-spectrometry | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:4932 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |