Intermittent hypoxia: changing energy metabolism patterns of golden pompano is beneficial for coping with hypoxia

However, dissolved oxygen in water is not always at the low concentrations that cause hypoxia, but it is possible to exhibit a state of intermittent fluctuation. The study used a low oxygen concentration (1.8 mg/L) to treat golden pompano (Trachinotus blochii) for 28 days (intermittent hypoxia, 2 hours per day) and systematically evaluated its metabolism indexes.

RNA-seq was used to construct a regulatory network to further elucidate the transcriptional regulation of the golden pompano metabolism under intermittent hypoxia. We found that the liver lactic acid content, as well as pyruvate kinase, hexokinase, and phosphofructokinase activities were elevated during the first 7 days, suggesting that anaerobic glycolysis was enhanced during the preceding period. In addition, the triglyceride, lipoprotein lipase, and carnitine palmitoyltransferase-1 levels in the liver were elevated after 14 days, suggesting that lipid utilisation was activated after 14 days.