Screening and bioinformatics analysis of characteristics genes of sepsis and aging based on GEO database

Finally, the BIDOS database was used to analyze the correlation between the expression level of characteristic genes and the survival time and APACHEII score of sepsis patients.Results There were 151 genes shared between sepsis and senescence, and the characteristic genes of TXN, CCL4, IL7 and SIRT1 were the common diagnostic markers of sepsis and senescence. The main gene sets that were up-regulated by the shared genes included cAMP signaling pathway, chemokine signaling pathway, PD-1 expression and PD-1 cancer checkpoint pathway, phospholipase D signaling pathway, PI3K-Akt signaling pathway, prolactin signaling pathway and other signaling pathways.

Immune filtration analysis showed that the high expression of CCL4 was positively correlated with the prognosis of sepsis, and CCL4 was significantly positively correlated with activated natural killer cells. TXN was significantly positively correlated with resting dendritic cells.Conclusion CCL4, TXN, IL7 and SIRT1 can be used as diagnostic biomarkers for sepsis and aging, and are closely related to the pathophysiological process of sepsis and aging.