<p><i>egl-2(G574E)</i> is a gain-of-function allele.</p>

(<b>A</b>) Time course of endogenous stress-induced sleep in animals of the indicated genotypes, plotted as the fraction of quiescent worms at each time point. Pre refers to before heat shock. Data were from three independent replicates, with 15–25 animals of each strain assayed on separate plates in each replicate.

Mean ± SEM are shown. (<b>C-E</b>) Quantification of sleep behavior shown as the fraction of quiescent worms for the indicated genotypes and conditions. All strains contain the transgene (HS::EGF) for conditional overexpression of EGF via 15-min heat shock (HS) at 33 °C to induce sleep.

Pre and post refer to before and 2 hours after heat shock, respectively. (C) The phenotype of <i>egl-2(tan200)</i> is similar to that of <i>egl-2(n2656, gof).</i> (D) <i>egl-2(tan200tan209[STOP-IN])</i> carries a STOP-IN cassette that disrupts the function of endogenous <i>egl-2(tan200[G574E])</i>. (E) <i>egl-2(G574E)</i> or <i>egl-2(+)</i> cDNA sequences were overexpressed in the ALA neuron of otherwise wild-type animals using an ALA-specific promoter (P<i>nlp-67</i>).

Each dot represents one independent assay containing 15–25 animals on a single plate. Mean ± SEM are shown. ns, not significant; *, p < 0.05; ****, p < 0.0001. One-way ANOVA with Dunnett’s multiple-comparisons test for post-heat shock samples, comparing each genotype with <i>egl-2(tan200)</i> (C-D) or wild type (E).</p>