Emerging infection and nosocomial transmission of carbapenem-resistant Klebsiella pneumoniae harboring blaNDM-21 in a neonatal intensive care unit

Genomic dataIllumina NovaSeq 6000 350-bp paired-end + Nanopore PromethION 10-kb long reads; hybrid assembly with Unicycler.Deposited in NCBI under BioProject PRJNA1398760 and PRJNA1265502; accession numbers JBTNPI000000000, JBTNPJ000000000, JBTNPK000000000, CP192299, CP192300, CP192301, CP192302.Phenotypic experimentsVitek-2 Compact GN334+XN04 MIC values.24-h growth curves (96-well, OD600 every 0.5 h, triplicate).Sedimentation ratio (SR) for mucoviscosity.Crystal-violet biofilm quantification (OD595).G. mellonella killing assay: 20 larvae/group, survival recorded every 6 h for 72 h; raw death times (h).Mouse survival: 5 mice/group, challenge 10^6 CFU, survival every 6 h for 72 h; raw death times (h).Organ bacterial loads: spleen, kidney, liver, lung at 24 h post-infection (CFU/organ, n=3).Original H&E histology whole-slide images.Bioinformatics filesAmino-acid sequences of NDM-1 to NDM-21.Neighbour-joining tree file (.meg).cgSNP and cgMLST distance matrices.Linear plasmid comparison SVG (pHS898-3 vs IncX3 reference).