The triterpenoid monomer from Butyriboletus roseoflavus ameliorates alcoholic liver disease related to Bifidobacterium animalis‑mediated suppression on hepatic Th17 cells

Here, Butyriboletus roseoflavus triterpenoid complex was prepared and column-purified, exerting prominent hepatoprotective effects against both acute and chronic ALD. Subsequent in vitro screening identified 16α‑Hydroxytrametenolic acid (HTA) as the principal active monomer for the hepatoprotective activity.

HTA treatment significantly alleviated alcohol‑induced hepatic steatosis, inflammation, and fibrosis, as evidenced by histopathological and biochemical analyses. Mechanistically, HTA markedly enriched intestinal Bifidobacterium animalis and upregulated the expression of phosphotransacetylase (Pta), a pivotal enzyme responsible for acetate biosynthesis. Both endogenous and exogenous acetate protected hepatocytes from alcohol-induced injury in vitro.