Raw sequencing data for Hernandez-Beltran et al (2026) "Forging an evolutionary individual from separate replicators"

This plasmid material was never passaged through yeast. 11109129250_MPB42093_lib00025_WPS00069049_FAZ13349_1.fastq.gz = ONT sequence data derived from Plasmid DNA extracted from ancestral yeast (MPB21532) and transformed into E. coli TOP10. 11109129250_MPB42094_lib00027_WPS00069049_FAZ13349_1.fastq.gz = ONT sequence data derived from Plasmid DNA extracted from derived yeast (cycle 70), Line 1 (MPB40417), and transformed into E. coli TOP10. 11109129250_MPB42095_lib00049_WPS00069049_FAZ13349_1.fastq.gz = ONT sequence data derived from Plasmid DNA extracted from derived yeast (cycle 70), Line 2 (MPB40418), and transformed into E. coli TOP10.

Folder: Yeast ONT Files: IC-A3608_MPB21532_libLAK3836_barcode07_FAB28259_a877d966_1.fastq.gz = ONT sequence data derived from genomic DNA extracted from MPB21532 (yeast BY4742 transformed with plasmids MPB20347 and MPB20848). IC-A3608_MPB40417_libLAK3837_barcode08_FAB28259_a877d966_1.fastq.gz = ONT sequence data derived from genomic DNA extracted from derived Line-1 populations at cycle 70 and sequenced (ONT). IC-A3608_MPB40418_libLAK3838_barcode09_FAB28259_a877d966_1.fastq.gz = ONT sequence data derived from genomic DNA extracted from derived Line-2 populations at cycle 70 and sequenced (ONT).

Folder: E_ coli with yeast derived plasmids_single clones ONT Files: MPB43209.rawdata.fastq.gz = ONT sequence data derived from plasmid DNA extracted from a single colony of E. coli TOP10 (MPB42094 – a derived Line 1 clone). Contains pChi with the FLP1 CDS Q389X mutation. MPB43214.rawdata.fastq.gz = ONT sequence data derived from plasmid DNA extracted from a single colony of E. coli TOP10 (MPB43214 – a derived Line 2 clone).

Contains the pChi with the FLP1 CDS frameshift at codon 78.