16S rRNA gene high-throughput sequencing data of rhizosphere microorganisms of broad bean grown at different altitudes in Qinghai Province

The V4 region of the bacterial 16S rRNA gene was selected as the amplification target, and PCR amplification was performed with universal primers 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTATCTAAT-3′). Pro Taq DNA polymerase was used for PCR reactions, with a total reaction volume of 20 μL. The PCR amplification program was set as follows: initial denaturation at 95 °C for 3 min; followed by 27 cycles of denaturation at 95 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 45 s; and a final extension at 72 °C for 10 min.

After quality verification of the amplified products, high-throughput sequencing was conducted on the Illumina MiSeq PE300 platform by Shanghai Majorbio Bio-pharm Technology Co., Ltd.