Data · dataset · 2024
Gas chromatography tandem mass spectrometry (GC-MS/MS) analysis of SCFAs
Listed in Teesside University Research Data Repository
The caecal contents (50 mg) were homogenized for 1 min with 50 μL of phosphoric acid (15%, v,v) and ether (400 μL) containing isocaproic acid (125 μg/mL, 100 μL) as an internal standard.
Description
After centrifugation for 10 min (12,000 rpm/min, 4℃), the supernatant was collected, and the concentrations of SCFAs in the samples were determined by GC/MS, equipped with an Agilent HP-Innowax capillary column (30 m × 0.25 mm; ID, 0.25 μm) in a trace 1310 GC system.
Helium was used as the carrier gas, with a flow rate of 1 mL/min. Injection was made in split mode at 10:1 with an injection volume of 1 μL and injector temperature of 250℃. The temperature of the ion source and GC/MS interface were 230°C and 250°C, respectively.
Read the rest (2 more)
The oven temperature was programmed to proceed from an initial temperature of 90°C to 120℃ at 10℃/min, and then to 150℃ at 5℃/min, and ultimately to 250℃ at 25℃/min, where it was held for 2 min. The mass spectrometer was operated in the electron impact ionization mode and selective ion monitoring mode with a collision energy of 70 eV. A mixture of SCFA standards (MilliporeSigma) that included acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, and caproic acid was used to prepare calibration curves at a concentration range of 0.02 to 500 μg/mL.
The concentration of total SCFAs was calculated as the sum of the measured concentrations of the 7 SCFA standards.
Links
Where it is published
- DOI doi.org/10.17632/z2ssm2pswg.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Mass spectrometry 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/z2ssm2pswg.1 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |