Data · dataset · 2026
Identification and bioactivity assessment of a protease inhibitor QUB-1833 from the skin secretion of Lithobates catesbeiana
Listed in ZivaHub and Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.17034/32629950.v1
As bioactive peptides in amphibians gained more and more attention, one of the common frogs, Lithobates catesbeiana, is meaningful to be studied.
Description
Its breeding history has lasted for decades, so people already know it quite well. But the research on the bioactive peptides in bullfrogs is not thorough.
Therefore, the research objectives in this study are isolation, identification, and bioactivity assessment of bioactive peptides from Lithobates catesbeiana skin secretions.<br><br>Using 'shotgun' cloning and DNA sequencing, the peptide sequences were obtained from secretions. Then, the peptide QUB-1833, whose amino acid sequence is SLRGCWTKSYPPQPCL, was selected as the research object. After synthesized by SPPS and purified by RP-HPLC and MALDI-TOF MS, pure peptide powder was got and further its biological activities were evaluated.
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The peptide QUB-1833 showed a very efficient inhibitory effect on trypsin. While it did not have any inhibitory activity against the selected three microorganisms (S. aureus, E. coli, and C. albicans) and two human cancer cell lines (U251-MG and HCT-116). This peptide has a very low hemolysis activity as well.<br><br>In the future, more in-depth research should be based on its good enzyme inhibitory effect, and improves its other bioactivities, so that it can be applied in practice.
Links
Where it is published
- DOI doi.org/10.17034/32629950.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75% · Sequencing 75%
Provenance · 4 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32629950 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32629950 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32629950 | 12 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/32629950 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |