miRNA‑sequencing project data

The samples cover multiple tissue types (roots, nodules, stems, etc.) and various treatment conditions (experimental groups including UL, UR, IL, IR and others). Each sample was subjected to total RNA extraction, small‑RNA library construction and high‑throughput sequencing on the Illumina platform.

The raw sequencing output met the targeted data volume, with data yields ranging from 9.95 M to 15.9 M reads across individual samples. The data are mainly provided in FASTQ format (raw sequencing reads). This dataset captures small RNA expression profiles of multiple soybean tissues under diverse treatment conditions, and can serve as fundamental data resources for studies including soybean small RNA identification, differential expression analysis and regulatory network reconstruction.