Data · dataset · 2026
Changes in the proteome of medicago sativa leaves in response to long-term cadmium exposure using a cell-wall targeted approach [* Cross-Reference *]
Listed in LORE
Accumulation of cadmium (Cd) shows a serious problem for the environment and poses a threat to plants.
Description
Plants employing various cellular and molecular mechanisms to limit Cd toxicity and alterations of the cell wall structure were observed upon Cd exposure. This study focuses on changes in the cell wall protein-enriched subproteome of alfalfa (Medicago sativa) leaves during long-term Cd exposure.
Plants grew on Cd-contaminated soil (10 mg/kg dry weight (DW)) for an entire season. A targeted approach was used to sequentially extract cell wall protein-enriched fractions from the leaves and quantitative analyses were conducted with two-dimensional difference gel electrophoresis (2D DIGE) followed by protein identification with matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time of flight (TOF/TOF) mass spectrometry.
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In 212 spots that showed a significant change in intensity upon Cd exposure a single protein was identified. Of these, 163 proteins are predicted to be secreted and involved in various physiological processes. Proteins of other subcellular localization were mainly chloroplastic and decreased in response to Cd, which confirms the Cd-induced disturbance of the photosynthesis.
The observed changes indicate an active defence response against a Cd-induced oxidative burst and a restructuring of the cell wall, which is, however, different to what is observed in M. sativa stems and will be discussed. ***This entry has been automatically imported via Infodoc(ASO) CSV by LIST harvest scripts. Please refer to doi.org/10.3390/ijms19092498 for the original and latest version of the dataset and data downloads***
Links
Where it is published
- Dataverse dataset page lore.list.lu/dataset.xhtml?persistentId=perma%3ALIST.0RMKZK ↗
landing page · from lore list lu
- Persistent identifier lore.list.lu/citation?persistentId=perma%3ALIST.0RMKZK ↗
DOI / persistent id · from lore list lu
Catalogue records · 1
- Dataverse API lore.list.lu/api/datasets/:persistentId/?persistentId=perma%3ALIST.0RMKZK ↗
metadata API · from lore list lu
Topics
- Stated by source
- Earth and Environmental Sciences
- From keywords
- Earth & Environmental Science · Life Sciences · Medicine & Health
- Inferred from text
- Mass spectrometry 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| LORE | perma:LIST.0RMKZK | 67 min ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].dataverse_subject:earth-and-environmental-sciences | source · lore list lu | connector:lore_list_lu@1.0.0 | /subjects |
| concepts[field].local:field:earth-environmental | mapping · lore list lu | connector:lore_list_lu@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · lore list lu | connector:lore_list_lu@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · lore list lu | connector:lore_list_lu@1.0.0 | /subjects |
| concepts[modality].local:modality:mass-spectrometry | enrichment · lore list lu | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · lore list lu | connector:lore_list_lu@1.0.0 | |
| description | source · lore list lu | connector:lore_list_lu@1.0.0 | /description |
| publication_date | source · lore list lu | connector:lore_list_lu@1.0.0 | |
| title | source · lore list lu | connector:lore_list_lu@1.0.0 | /name |
| updated_date | source · lore list lu | connector:lore_list_lu@1.0.0 | |
| version_label | source · lore list lu | connector:lore_list_lu@1.0.0 |