Table 5_The shifts of submucosal microbiota in treated patients with aggressive periodontitis.docx

Introduction<p>This study aimed to investigate the evolution of the submucosal microbiota and compare the submucosal and subgingival microbiota in patients with aggressive periodontitis.</p>Methods<p>Patients with aggressive periodontitis underwent initial periodontal and surgical therapy, followed by implant restoration. Samples were longitudinally collected from the teeth and implants before the initial therapy (V0), before implantation (V1), one month after abutment connection (V2), one month (V3) and one year (V4) after restoration.

The 16S rRNA V3-V4 region of subgingival and submucosal samples was analyzed using the MiSeq platform.</p>Results<p>A total of 108 subgingival pooled samples and 24 submucosal pooled samples from 6 patients were sequenced to generate 3,129 operational taxonomic units (OTUs) capturing 12 phyla, 27 classes, 49 orders, 92 families, 195 genera, and 554 species. A considerable microbial shift was observed from 1 month after abutment connection to 1 month after loading (V2 to V3); however, this shift slowed during the remaining study period.