Data · dataset · 2025
Brucella proline racemase protein A targets Tpl2 to promote IL-10 secretion for establishment of chronic infection
Listed in ScienceDB
This dataset includes the IL-10 concentration in macrophages after stimulation with rRomA, rOMP25, rwadC, and rPrpA proteins in vitro and in vivo.
Description
It also includes the IL-10 concentration in macrophages aftertransfection with varying doses of the pcDNA3.1-prpA plasmid. All IL-10 concentrations were determined using ELISA kits.
Additionally, the dataset contains data on the number of cells induced by PrpA, as well as the proliferation rate of macrophages (evaluated using CCK-8 kits). Furthermore, the dataset includes data on the intracellular survival of M5-90, the M5-90 prpA mutant, and M5-90 prpA-c in murine macrophages. It also comprises proteomics data of murine macrophages infected with M5-90 and the M5-90 prpA mutant, along with results from molecular dynamics simulations of the PrpA and Tpl2 proteins.
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Finally, the dataset includes the following: 1) Spleen colony-forming units (CFUs) of mice inoculated with M5-90 and the M5-90 prpA mutant at 30 days post-inoculation; 2) Serum levels of Brucella-specific IgG antibody, IFN-γ, TNF-α, and IL-10 (evaluated using ELISA kits) at 30 and 45 days post-inoculation; 3) Flow cytometry analysis data of splenic lymphocytes from mice immunized with M5-90 and the M5-90 prpA mutant at 30 and 45 days post-immunization; 4) CFU counts in the spleen from challenge experiments.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.28794 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Mass spectrometry 65%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.28794 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |