Data · dataset · 2025
Original data (CT values) of RT-qPCR.
Listed in ScienceDB
The T. urticae mites, collected as described in section 2.6, were used for RNA extraction, following the protocol outlined in section 2.3.
Description
Total RNA was reverse transcribed into complementary DNA (cDNA) using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Dalian, China). Based on the gene sequences obtained from cloning, specific primers for RT-qPCR were designed using the Primer3 Input online tool (refer to Table S1).
For normalization of gene expression levels, the α-tubulin gene (GenBank Accession: JN881327.1) was selected as the internal reference gene. RT-qPCR was conducted on an ABI QuantStudio 5 Real-Time PCR System with Hieff UNICON qPCR SYBR Green Master Mix (Yeasen, Shanghai, China). Three technical replicates were performed for each biological replicate.
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Gene expression levels were quantified using the 2-ΔΔCt method
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.22345 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Computed tomography 50% · Tabular 65%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.22345 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:ct | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (50%) |
| concepts[modality].local:modality:tabular | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |