Data · dataset · 2026
SINGLE NUCLEOTIDE VARIATION ANALYSIS OF THE GCRV-II S10 AND S11 SEGMENTS BASED ON THIRD-GENERATION SINGLE-MOLECULE NANOPORE SEQUENCING
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To investigate the genetic variation and evolutionary patterns of genotype II grass carp reovirus (GCRV-II), in this study, we analyzed 20 GCRV-II isolates collected from Guangdong, Hunan, Jiangxi, and other provinces from 2017 to 2025.
Description
Third-generation single-molecule nanopore sequencing was employed to perform single nucleotide variant (SNV) analysis on the S10 and S11 genome segments of GCRV-II. The number and density of variant sites, together with the Shannon entropy diversity index, were used to evaluate differences in quasispecies genetic diversity among different GCRV-II strains.
The results showed that varying degrees of nucleotide variation occurred in both the S10 and S11 segments of the 20 GCRV-II isolates, with each variant site containing at least two nucleotides and nucleotide transitions predominating. Overall, the S10 segment exhibited a higher level of variation than the S11 segment. SNV density ranged from 0 to 144.1 SNVs/kb in the S10 segment and from 0 to 21.4 SNVs/kb in the S11 segment.
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Shannon entropy analysis revealed marked differences in quasispecies genetic diversity among GCRV-II isolates collected at different times and from different geographical regions, suggesting that quasispecies variation among GCRV-II strains may be associated with geographic origin or epidemic period. Phylogenetic analysis further demonstrated clear evolutionary divergence among the 20 GCRV-II isolates. Based on third-generation single-molecule nanopore sequencing, this study compared quasispecies genetic diversity at the single-nucleotide level in the S10 and S11 segments of GCRV-II strains from different sampling years and geographical origins.
These findings provide a foundation for further elucidating the genetic variation patterns and evolutionary mechanisms of GCRV-II, and offer theoretical support for vaccine candidate selection and epidemiological surveillance.
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Where it is published
- DOI doi.org/10.57760/sciencedb.38666 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Medicine & Health · Social Science
- Inferred from text
- Bioinformatics and computational biology 74% · Sequencing 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.38666 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3102 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (74%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
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| concepts[field].local:field:medicine-health | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |