Omics · study · 2026
High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation with CsdA or PNPase in E.coli
Listed in NCBI GEO
Our results show the RNA transcripts in complex with the helicase CsdA or the exoribonuclease PNPase.
Description
We used CsdA or a catalytically inactive variant of PNPase (PNPase D492G) as bait to capture enriched substrates bound by the enzymes. Sequencing of RNA isolated by crosslinking immunoprecipitation with these enzymes identified numerous transcripts bound to CsdA or PNPase.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315071 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315071 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1394967 ncbi.nlm.nih.gov/bioproject/PRJNA1394967 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE315071 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |