Table · dataset · 2024
MST_dataset: The K+ exchange antiporter 3 senses the chloroplast energy status to synchronize photosynthesis
Listed in Edmond
The N-terminal His-tag of the regulatory C-terminus of KEA3 or a mutant carrying a G65>A point mutation was labelled with the RED-tris-NTA kit (NanoTemper Technologies GmbH, Munich, Germany).
Description
After 10 minutes incubation on ice, 50 nM of protein sample in PBS together with the indicated concentrations of nucleotides were loaded into standard Monolith NT.115 capillaries (NanoTemper Technologies GmbH, Munich, Germany). TRIC measurements were performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany) at ambient temperature of 23 °C. Instrument parameters were adjusted to 80 % LED and 60 % MST power.
TRIC as differences in normalized fluorescence was calculated between the initial fluorescence and 1.5 s after the onset of the laser. 10 mM nucleotide stocks were prepared freshly in PBS pH 8.0 directly before the experiment and the pH was checked.
Links
Where it is published
- Dataverse dataset page edmond.mpg.de/dataset.xhtml?persistentId=doi%3A10.17617%2F3.75PWRF ↗
landing page · from edmond mpdl mpg de
- DOI doi.org/10.17617/3.75pwrf ↗
DOI / persistent id · from edmond mpdl mpg de
Catalogue records · 1
- Dataverse API edmond.mpg.de/api/datasets/:persistentId/?persistentId=doi%3A10.17617%2F3.75… ↗
metadata API · from edmond mpdl mpg de
Topics
- From keywords
- Astronomy & Astrophysics · Chemistry · Computer Science & AI · Earth & Environmental Science · Economics & Finance · Engineering · Engineering · Humanities · Life Sciences · Life Sciences · Materials Science · Medicine & Health · Ocean & Atmospheric Science · Physics · Social Science
- Inferred from text
- Biochemistry and cell biology 73%
Provenance · 2 source records, 24 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Edmond | doi:10.17617/3.75PWRF | 10 d ago | JSON v1 |
| Edmond | doi:10.17617/3.75PWRF | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
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