Allele and haplotype frequencies of the Czech Graves´disease patients

We analyzed the HLA- DRB1 , HLA- DQA1 and HLA- DQB1 allelic groups in 206 retrospectively observed Czech patients who had been diagnosed with Graves' disease according to hormonal profile (low/suppressed TSH with simultaneously elevated freeT4, freeT3, presence of thyrotropin receptor antibodies (TRAK)) as well as sonographic examination of the thyroid gland. Patients were recruited during the years 2017-2020 mainly from the outpatients of the Institute of Endocrinology in Prague.

These patients are continuously monitored at the Institute, which gives us detailed long-term or even lifelong retrospective information on the status of their disease. Next generation sequencing performed on a MiSeq (Illumina) was used for haplotyping. In the three HLA genes ( DRB1 , DQA1 and DQB1 ), exon 2 was amplified, since this part of the HLA molecule determines its antigenic properties.