Data · dataset · 2025
Sequencing of mRNA, lncRNA, and circRNA in LLC Cells Treated with Ophiopogonin D
Listed in ScienceDB
Lung cancer, especially non-small cell lung cancer (NSCLC), is the leading cause of cancer-related deaths worldwide.
Description
Natural products play a crucial role in drug development, and most current therapies are derived from these ingredients. Ophiopogon D (OP-D) has received widespread attention due to its broad anti-cancer properties, including the ability to inhibit cell proliferation and induce cell apoptosis. In this study, we performed whole transcriptome sequencing on LLC cells treated with OP-D. Cultivate LLC cells using standard methods and use TRIzol ® Extract total RNA using reagents.
Evaluate RNA quality using Agilent 5300 Bioanalyzer and quantify using NanoDrop ND-2000. Construct sequencing libraries using only high-quality RNA samples (OD260/280=1.8-2.2, OD260/230 ≥ 2.0, RQN ≥ 6.5, 28S: 18S ≥ 1.0,>1 μ g). We used Illumina Stranded Total RNA Prep and Ribo Zero Plus for library preparation, generating ribosomal RNA removal libraries including mRNA, lncRNA, and circRNA information.
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These libraries were sequenced on the Illumina NovaSeq Xplus platform at Majorbio in Shanghai, and a large dataset was uploaded in FASTQ format. The main findings indicate that OP-D processing resulted in significant inter group differentiation as shown in principal component analysis (PCA). Differential expression analysis showed significant downregulation of lncMALAT1, confirming MALAT1 as a significant lncRNA. In addition, mRNA analysis showed that OP-D treatment inhibited key features of malignant tumors, including metastasis and proliferation, highlighting its extensive inhibitory effect on carcinogenic processes.
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Where it is published
- DOI doi.org/10.57760/sciencedb.28998 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Cancer 75% · RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.28998 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
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| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |