Data · dataset · 2026
Efficacy of docetaxel against Toxoplasma gondii in vitro
Listed in ScienceDB
Fresh RH tachyzoites (approximately 2×107 parasites) were incubated with docetaxel (5 nM) or 0.1% DMSO (vehicle) for 12 h at 4 ℃.
Description
Total RNA was extracted using TRIzol (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol. RNA quality was determined using an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).
RNA-Library preparation and sequencing were conducted by Novogene (Beijing, China). Sequencing was performed using the Illumina platform (Illumina, San Diego, CA, USA). Clean reads were aligned to the T. gondii reference genome (GenBank accession number: PRJNA28893) using HISAT2 software. We used the DESeq to perform differentially expressed gene (DEG) analysis (P-value < 0.05).
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GO enrichment analysis of differentially expressed genes was implemented by the cluster Profiler R package, in which gene length bias was corrected (Yu et al., 2012). GO terms with corrected P-value < 0.05 were considered significantly enriched by differential expressed genes. We used cluster Profiler R package to test the statistical enrichment of differential expression genes in KEGG pathways.
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Where it is published
- DOI doi.org/10.57760/sciencedb.36081 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.36081 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |