Heparan sulfate co-immobilized with cRGD ligands and BMP2 on biomimetic platforms promotes BMP2 mediated osteogenic differentiation. Figure 1 c and g

Two experiments realized and used for quantification, as well as the experiment shown in Figure 1c of the paper Sefkow-Werner et al., 2020, (submitted), are shown here. The quantification of the three independent experiments shows the average ± SEM in Figure 1d of the cited paper. Figure 1g: Western blot of p-SMAD 1/5/9 expression of C2C12 cells plated for one hour on cRAD platforms and exposed to the same concentration of sBMP2 (6 nM).

As control, C2C12 were plated on a plastic petri-dish (TCPS) with or without sBMP2. One hour after plating cells were lysed and the level of p-SMAD 1/5/9 plotted and normalized to the housekeeping gene GAPDH. The two experiments realized and used, together with the blot shown in Figure 1g of the paper Sefkow-Werner et al., 2020 (submitted), are shown here.

The quantification of the average ± SEM of the three independent experiments is shown in Figure 1h.