C-type natriuretic peptide pre-maturation culture affects cytoplasmic maturation in bovine oocytes

Modulation of ribonucleic acid (RNA) transcripts, lipids, mitochondria, and other molecules during maturation influences development significantly. To improve current maturation systems, inclusion of pre-maturation culture has been investigated to delay resumption of meiosis and allow appropriate progression of cytoplasmic maturation using C-type natriuretic peptide (CNP).

In this study, the transcriptome and molecular fingerprint of germinal vesicle, conventional IVM, and CNP-IVM oocytes were compared to identify transcripts and associated pathways altered by CNP treatment. There were similar pathways associated with upregulated transcripts in both types of mature oocytes involved with mitochondrial function and translation. Oocytes that were matured under conventional conditions contained transcripts associated with messenger RNA surveillance, Polycomb repressive complex, and reactive oxygen species.