Supplementary data – Catfish treatment for Thaparocleidus vistulensis infection – Wan Sajiri, 2026

This study aimed to describe the in vivo efficacy of specific anthelmintics, such as Praziquantel (PZQ) and Mebendazole (MBZ) (administered as bath treatments), against the infection. Initial toxicity tests exposed juvenile European catfish to PZQ and MBZ at concentrations of 0, 10, and 20 mg/L (24 h bath treatment) followed by a 14 days post-treatment (dpt) observation period.

These tests demonstrated that European catfish could tolerate 10 mg/L of PZQ and MBZ without any signs of stress. In vivo treatment experiments were conducted by exposing experimentally infected catfish to 0, 1, 5, and 10 mg/L of PZQ or MBZ, after which fish were examined 1, 7, and 14 dpt. PZQ at the highest concentration eliminated 100% of parasites from the gills of juvenile European catfish within 24 h.