Role of LIN-15A on the binding of LIN-15B and LIN-35

Experiments were conducted in wild-type animals and in lin-15A(fp31), lin-15B(n744), and lin-35(n745) null mutants. All three factors showed highly overlapping binding patterns, predominantly at promoter regions, and shared target genes enriched for metabolic and developmental pathways.

Comparative analyses revealed reciprocal effects on binding between LIN-35 and LIN-15B, while loss of LIN-15A primarily resulted in increased LIN-35 and LIN-15B occupancy at a subset of loci, suggesting that LIN-15A modulates DREAM complex binding.