RNA-seq analysis of spiral ganglion neurons treated with cisplatin and dimethyl fumarate to elucidate otoprotective mechanisms

Spiral ganglion neurons (SGNs) were divided into two treatment groups: cisplatin alone and cisplatin combined with DMF. Gene expression profiling was performed using high-throughput RNA-sequencing.

Differential expression analysis identified a set of significantly upregulated and downregulated genes in the DMF combination group compared to the cisplatin-only group. Pathway enrichment analysis highlighted significant alterations in autophagy and mitophagy pathways, suggesting that DMF may alleviate cisplatin-induced damage by modulating these processes in SGNs. This dataset provides the raw sequencing reads (FASTQ) and gene count matrix, serving as a valuable resource for investigating drug-induced ototoxicity and protective therapeutic strategies.