Characteristics of microsatellite loci developed for <i>Leucogeranus leucogeranus</i>

<p dir="ltr">The table contains characteristics of primer sequences, repeat motif, multiplex panel, dye, annealing temperature, chromosome, gene and allelic size range of newly developed microsatellite (SSR) loci in the Siberian (<i>Leucogeranus leucogeranus</i>)<i>, </i>Eurasian (<i>Grus grus</i>)<i> </i>and White-naped (<i>Antigone vipio</i>) cranes and PCR program used for amplification<i>. </i>Microsatellite loci were developed for the first time for the Siberian crane based on whole-genome data from this species (Chen et al., 2025; 2026) to analyze the genetic diversity of wild and captive populations.

The loci are also shown to be useful in applications to other crane species such as identification of species, hybrids, populations and individuals and comparison of their genetic diversity using a standardized set of markers. Crane species from other genera, <i>Grus</i> and <i>Antigone, </i>with no species-specific SSR loci available, were chosen to test the cross-amplification abilities. For ease of use, the developed primers were organized into three multiplex panels targeting loci located on different chromosomes.