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Data · dataset · 2026

<p><i>egl-2(G574R)</i> is a gain-of-function allele and disrupts EGF-induced sleep.</p>

Listed in UCL Research Data Repository

<p>(<b>A-B</b>) Quantification of sleep behavior as the fraction of quiescent worms for the indicated genotypes and conditions.

Description

All strains contain the transgene (HS::EGF) for conditional overexpression of EGF via 15-min heat shock (HS) at 33 °C to induce sleep. Pre and post refer to before and 2 h after heat shock, respectively. <i>egl-2(tan200)</i> and <i>egl-2(gk593448)</i> were used as <i>egl-2(G574E)</i> and <i>egl-2(G574R</i>), respectively.

Homozygous (A) and heterozygous (B) animals were assayed to compare the sleep phenotype. Each dot represents one independent assay containing 15–25 animals on a single plate. Mean ± SEM are shown. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

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One-way ANOVA with Tukey’s multiple-comparisons test for post-heat shock samples. (<b>C</b>) Time course of endogenous stress-induced sleep in animals of the indicated genotypes shown as the fraction of quiescent worms at each time point. Pre refers to before heat shock.

The <i>egl-2(gk583448)</i> allele was used for <i>egl-2(G574R)</i>. Data were collected from three independent replicates, with 15–25 animals of each strain assayed on separate plates in each replicate. Mean ± SEM are shown. ns, not significant; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Two-tailed unpaired Student’s t-test for comparing wild type and <i>egl-2(gk583448)</i> mutants at each time point. (<b>D-E</b>) Representative images and quantification of endogenous EGL-2 protein expression in the ALA neuron using a split-GFP strategy. (D) Top: <i>egl-2(+)::7x gfp11</i>; Middle: <i>egl-2(G574E)::7x gfp11</i>; bottom: <i>egl-2(G574R)::7x gfp11</i> animals.

All worm strains also carried the transgene <i>ALAp::gfp1–10</i> for ALA-specific expression of GFP1–10. White dashed ovals outline the ALA neuron cell body. A, anterior; D, dorsal.

Scale bar, 20 μm. (E) Numbers shown on the bars indicate the total number of animals imaged for each genotype. ns, not significant; *, p < 0.05; ****, p < 0.0001. One-way ANOVA with Tukey’s multiple-comparisons test.</p>

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From keywords
Biochemistry and cell biology · Caenorhabditis elegans · Cancer · Earth & Environmental Science · Genetics · Life Sciences · Medicine & Health
Inferred from text
Image 65%
Provenance · 1 source records, 13 field assertions
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UCL Research Data Repositoryoai:figshare.com:article/340495399 d agoJSON v1
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