Table 1_Epidemiological investigation and whole-genome genetic characterization of duck circovirus in Shandong Province of eastern China.docx

Riemerella anatipestifer and duck parvovirus were identified as the dominant co-infecting pathogens. Genomic analysis showed that the full-genome lengths of the three complete DuCV genome sequences ranged from 1988 bp to 1993 bp. The three sequenced DuCV genome sequences belonged to the DuCV-1b and DuCV-2c sub-genotypes.

Notable differences were observed in the N-glycosylation site and 3D structure of the cap proteins between the two genotypes. The three novel DuCV genome sequences shared 56.40–99.79% nucleotide sequence identity with global reference DuCV sequences. Phylogenetic trees of DuCV based on the whole genome, Cap gene, and Rep gene were constructed using reference strains from around the world.</p>Conclusion<p>This study provides updated epidemiological survey data and genetic information on DuCV in Shandong Province, China, which provides reference data for further research on the prevention and control of DuCV infection.</p>