<p>The characteristics of NET formation captured by a fluorescence microscope (original magnification, 200 × ; scale bars = 50 μm) showing neutrophils co-cultured with PMA (100 ng/mL; positive control) or <i>Pythium</i> zoospores.</p>

The percentage of NET-releasing neutrophils was quantified relative to the negative control (unstimulated neutrophils) (C), which was expressed as the number of MPO-positive cells (D) and NE-positive cells (E) per 100 neutrophils.

The data are shown as the mean ± S.E.M., ****, <i>p-value <</i> <i>0.0001; **, p-value = 0.0012; *, p-value = 0.0219; ns, p-value > 0.0001</i> MOI 1 of β-thalassemia patients vs. MOI 1 of healthy controls, using One-way ANOVA analysis (n = 6/group). Abbreviations are as follows: NE, neutrophil elastase; MPO, myeloperoxidase; NET, neutrophil extracellular trap; DAPI, 4′,6-diamidino-2-phenylindole; PMA, 12-myristate 13-acetate; Neu, neutrophils; Neu (H), neutrophils isolated from healthy individuals; Neu (Pt), neutrophils isolated from β-thalassemia patients; MOI 1 (H), co-incubation of neutrophils isolated from healthy individuals with <i>P. insidiosum</i> zoospores at a multiplicity of infection (MOI) of 1; MOI 1 (Pt), co-incubation of neutrophils isolated from β-thalassemia patients with <i>P. insidiosum</i> zoospores at MOI of 1.</p>