<p>A peptide vaccine containing bacterial epitopes of CD4⁺ T cells is immunogenic in mice.</p>

Note

The unequal group sizes were due to the limited availability of animals. (C-E) Number of cells producing IFN-γ, IL-2 and TNF-α in splenocytes following stimulation with a subset of peptides contained in the vaccine. Splenocytes were isolated 19 days after administration of the booster dose and pooled from groups of 3 mice.

Data from individual wells for each condition and the corresponding mean values are shown. For all assays, peptides were used at a final concentration of 5 µM, and control wells without peptide (unstim) or with a negative peptide (YAIAVNDLGTEYVHR) were included. Significant differences compared to the control (unstim) were identified using two-way ANOVA followed by Dunnett’s post hoc test.

Asterisks indicate p-values (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). This experiment was performed once.</p>