iTRAQ-Based Proteomics Analysis of Response to Solanum tuberosum Leaves Treated with the Plant Phytotoxin Thaxtomin A

The S. tuberosum group Phureja DM1–3 were treated with TA and total proteins were extracted from five different plants (pooled together as one sample). The proteomic changes in potato leaves treated with TA were determined using the iTRAQ technique. Protein identification was performed using ProteomeDiscovererTM 2.2 aligned to the NCBInr database (ncbi.nlm.nih.gov/; accessed on 18 January 2019) and SwissProt/UniProt database (uniprot.org/; accessed on 18 January 2019) by DIAMOND.

Peptide false discovery rate (FDR) analysis ≤0.01 was the filtering parameter. DEPs were identified using a FC (fold change) threshold, and p-values of <0.05 were significant. FC > 1.2 was considered upregulated, and FC < 0.83 was considered downregulated.