Expression of diabetes-related genes in rodent β-cell line (RIN-m) treated with monocarbonyl curcumin analogues (C66 and B2BrBC) in presence or absence of streptozotocin – PCR array data

Cyril and Methodius University in Skopje, Macedonia by substituting benzaldehyde with cyclohexanone in a base catalyzed aldol reaction. RIN-m rat β-cells (ATCC, Cat. # CRL-2057) were grown in RPMI-1640 medium (ATCC, Cat. # 30-2001) supplemented with 10% FBS (VWR, Cat. # 89510-186) and antibiotic/antimycotic mixture (MP Biomedicals, Cat. # 1674049) in incubator with standard conditions (5% CO2 and temperature of 37 °C).

For experiments, 0.3 x 10(6) cells were seeded in 6-well plates with tissue culture growth media. The following day the media was replaced with serum-free media supplemented with 1.5 mM of streptozotocin (Sigma-Aldrich Cat. # 85882), 50 µM of C66, or B2BrBC dissolved in DMSO (VWR, Cat. # N182), and combination of streptozotocin with C66 or B2BrBC, for 72 hours. Total RNA was extracted using TRIzol reagent (Ambion, Cat. # 15596018).