Transcriptomic profiling of rat supraspinatus muscle after tissue-adaptive flexible ultrasound patch treatment in a rotator cuff injury model

Bulk RNA-seq was performed on supraspinatus muscle tissues from control and TA-FUP-treated rats to characterize treatment-induced transcriptional changes. Differential expression and pathway analyses showed that TA-FUP broadly reprogrammed the degenerative transcriptomic profile of injured supraspinatus muscle. Genes and pathways associated with adipogenesis, lipid transport, lipid metabolic process, extracellular matrix organization, collagen fibril organization, immune response and inflammatory response were downregulated after TA-FUP treatment.

Gene set enrichment analysis further suggested enhanced fatty acid degradation and suppression of adipocyte-associated lipid remodeling, ECM-receptor interaction and NF-κB signaling. These data provide transcriptomic evidence that TA-FUP attenuates supraspinatus muscle degeneration after rotator cuff injury by suppressing adipogenic, fibrotic and inflammatory remodeling programs.