Replication Data for: Phase Retention Control in Preparative Centrifugal Partition Chromatography

It now allows to measure the retention inside the rotor during operation and to use this information for its closed loop-loop control via redosing of stationary phase. This results in maintaining the separation performance over prolonged operating times, which is shown by separating salicylic acid and D(+) carvone over a period of approximately 8 hours using a preparative rotor. At the same time, we showed that the specific solvent consumption can be decreased by approximately 53 %, when redosing of stationary phase is applied.

After its functionality has been proven, the CC is further developed. With the improved design, its applicability in both ascending and descending mode is successfully demonstrated. Furthermore, the performance of the CC is investigated for a different rotor volume.

Finally, the advantage of redosing during separation is highlighted for systems in which severe bleeding occurs, as demonstrated by the separation of vanillin and coumarin.