Evaluation of bioactivity of QUB1977 from the skin secretion of the frog, Phyllomedusa camba

It is proven that many of these peptides exhibit antimicrobial, anticancer, antiviral and trypsin inhibitory activities and therefore their potential promising therapeutic value has drawn great attention from numerous researchers<br>In this study, a novel antimicrobial peptide was isolated and identified from the skin secretion of the frog, Phyllomedusa camba.

A “shotgun” cloning technique was used to select the biosynthetic precursor sequence and a cDNA library was constructed for amplification of the target sequence. The peptide was isolated and identified using HPLC, MALDI-TOF mass spectrometry.<br>The synthetic replicate exhibited a broad spectrum antimicrobial activity against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli and the yeast Candida albicans at concentrations of 4, 32 and 4 µM, respectively.