Data · dataset · 2026
QUB-1951: a bioactive peptide from the skin secretion of the Chinese piebald Odorous frog, Odorrana schmackeri
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32862986.v1
Peptides derived from the secretion of various frogs and toads show a great diversity and potential for discovery and development of novel therapeutics.
Description
In recent years, peptide-based drugs have been in the spotlight due to their high therapeutic potential combining high efficacy with low toxicity. <br><br>In this research, a bioactive peptide was obtained from the skin secretion of the Chinese piebald odorous frog (Odorrana schmackeri).
The precursor encoded cDNA was identified by using “shotgun” cloning, with the amino acid sequence of putative mature peptide, AVNIPFKVHFRCKAAFC. In order to obtain sufficient peptides to carry out the bioactive assays, solid phase peptide synthesis (SPPS) was used to synthesize. The molecular weight was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and purified by reverse phase-high performance liquid chromatography (RP-HPLC).
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This synthetic peptide was then subjected to functional assays, including antimicrobial assay, trypsin inhibition assay haemolysis assay, and cell viability assay. The results showed that this peptide had the ability to inhibit trypsin with Ki value of 1.97 μM, but neither antimicrobial nor severe haemolytic activity were detected. It was the first time to report a novel kunitz inhibitor peptide discovered from the frog secretion, which provides new insight of unique trypsin inhibitors from natural products.<br>
Links
Where it is published
- DOI doi.org/10.17034/32862986.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Mass spectrometry 75%
Provenance · 3 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32862986 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32862986 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32862986 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |