Bioactivity assessment of peptide QUB1471 from the skin secretion of the Chinese torrent frog (Amolops wuyiensis)

Since ancient times, substances derived from amphibian skins have been recognised to possess various medicinal properties. In recent years, bioactive peptides from amphibian skin secretions have attracted much attention for their profound significance in providing clues directly towards novel drug development, for better understanding of miscellaneous physiological and pathological processes, for elucidation of phylogenetic relationships, and for improved taxonomy.<br>In this study, the isolation and identification of bioactive peptides from the skin secretion of Chinese torrent frog, Amolops wuyiensis, were performed through the genomic approach shotgun cloning.

The mRNA was isolated from the skin secretion and was used to construct cDNA library by reverse transcription. The target cDNA of interest was amplified and sequenced using molecular cloning. Once the sequence was confirmed, the peptide was chemically synthesised, and biological activity screening was performed subsequently.