Data and analysis code for: Molecular mechanics of smooth muscle contraction and relaxation modulated by caldesmon

This dataset contains the experimental data and analysis scripts supporting the findings presented in the manuscript: "Molecular mechanics of smooth muscle contraction and relaxation modulated by caldesmon." Dataset Components Analysis Code: Provided as a single .zip file containing Python scripts for data extraction, mathematical modeling, and statistical analysis. Includes a detailed ReadMe file. Experimental Data: Uploaded as separate .zip archives (organized by experiment).

These archives contain raw microscopy videos and intermediate .mat files generated by the MATLAB-based IVMA analysis software. Directory Structure & Experimental Descriptions The data and scripts are organized into the following functional directories based on the experimental assays performed: actin_biotinylation_and_CaD: In vitro motility assays (IVMA) investigating the effects of actin biotinylation on velocity and motile fraction in the presence of CaD (Figure S2). analytical_estimation_of_dePhos_rate: Mathematical estimation of myosin dephosphorylation rates with and without caldemson (CaD) (Figure S3). mixture_motility_assays (Parts 1 & 2): Assays using varying ratios of phosphorylated and unphosphorylated myosin with and without CaD.