SpxA1 and SpxA2 Function as a Stoichiometry-Dependent Regulatory Rheostat Governing Virulence Gene Expression in Group A Streptococcus — RNA-seq dataset

To define the transcriptomic consequences of SpxA1 and SpxA2 loss, we performed strand-non-specific paired-end RNA-seq in the wild-type emm3 GAS strain MGAS10870 and isogenic deletion mutants (deltaSpxA1 and deltaSpxA2) grown to mid-exponential phase in Todd-Hewitt broth supplemented with 0.2% yeast extract (THY).

Differential expression analysis identified distinct transcriptomic signatures for each paralog, with SpxA1 primarily influencing oxidative stress response genes and SpxA2 modulating CovR-regulated virulence factor-encoding genes. These data contribute to a multi-omic analysis including DIA proteomics, NanoString nCounter, and CovR ChIP-exo that defines the regulatory roles of SpxA1 and SpxA2 in GAS pathogenesis.