Data · dataset · 2026
<p>NOXA is a constitutive pro-apoptotic signal in PEL cell lines.</p>
Listed in UCL Research Data Repository
<p>A. Model to link the oncogenic functions of MARCHF5 and MCL1.
Description
We propose that in PEL cell lines, MARCHF5 cooperates with MCL1 function to induce the proteasomal turnover of the MCL1 antagonist NOXA. B. mRNA expression of BH3-only proteins in PEL cell lines from Cell Model Passports.
C. Western blots of BH3-only proteins that were targeted for Cas9 KO in BC-3 cells. D. Experimental flow chart of the Fitness Competition Assay. BC-3 Cas9 cells were transduced with a lentivirus encoding the control g<i>AAVS1</i> and an iRFP670 fluorescent protein or a second lentivirus encoding the targeting gRNA and an eGFP reporter.
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Equal amounts of control iRFP670 cells were mixed with targeted eGFP cells at the beginning of the growth curve. Fitness indices as reflected by the ratio of eGFP to iRFP670 (eGFP:iRFP670) were measured over 15 days by flow cytometry. E. Fitness indices for each BH3-only KO competition relative to day 0.
Statistical differences were calculated using two-way ANOVA with Dunnett’s multiple comparison post-hoc test (n = 3). Adjusted <i>p</i> values reflect post-hoc comparisons between gSAFE4501 to BH3-only protein gRNA. Error bars, standard error of the mean.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.ppat.1014665.g006 ↗
DOI / persistent id · from rdr ucl ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from rdr ucl ac uk
Topics
- From keywords
- Biochemistry and cell biology · Cancer · Earth & Environmental Science · Genetics · Infectious diseases · Life Sciences · Medicine & Health · Virology
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UCL Research Data Repository | oai:figshare.com:article/34049132 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[disease].local:disease:cancer | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[field].anzsrc:field:310706 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:320211 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:group:3101 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| description | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| title | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/title |